Journal of ethnopharmacology · 2025
Antinociceptive activity of 80 % methanolic leaf extract of Pterolobium stellatum (Forssk.) Brenan (Fabaceae) in mice.
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Salile Samson Sahile, Woldamo Biniyam Abera, Zewdie Hana Biruk, Ali Hana Saif, Kediso Teketel Eristu, Aysa Biruk Seifu, Orjino Teferra Abula, Abiye Alfoalem Araba
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Pterolobium stellatum (P. stellatum) is traditionally used for managing pain in Ethiopia.
AIM OF THE STUDY: To investigate the anti-nociceptive effects of 80 % methanol leaf extract of P. stellatum in mice.
MATERIALS AND METHODS: The central and peripheral analgesic effects of the extract were assessed at doses of 100, 200, and 400 mg/kg using the hot plate (HPT) and formalin tests (FT) in mice. Phytochemical screening was also performed, including a qualitative evaluation of 14 compounds and the quantification of total content of alkaloids, flavonoids, and phenols. Additionally, an acute toxicity test was performed to assess the safety of the plant extract. Data were analyzed using a one-way analysis of variance (ANOVA) followed by Dunnett's t-test, with statistical significance set at p < 0.05.
RESULTS: The P. stellatum extract was safe at 2000 mg/kg with no observed toxicity. Phytochemical screening confirmed the presence of alkaloids, flavonoids, phenols, saponins, tannins, sterols, terpenoids, coumarins, and fats/oils. Quantitatively, the extract demonstrated measurable levels of total flavonoids, phenols, and alkaloids. Phytochemical quantification showed flavonoids 893.7 ± 10.49 μg/g, phenols 789.76 ± 55.15 μg/g, and alkaloids 222.64 ± 17.40 μg/g (mean ± SE, n = 3). In the hot plate test (HPT), all doses significantly increased pain-reaction latency (p < 0.05). In the formalin test (FT), the 200 mg/kg and 400 mg/kg doses produced significant inhibition of paw-licking behavior in the inflammatory (late) phase (p < 0.05), while morphine inhibited both phases (p < 0.05).
CONCLUSION: The methanolic leaf extract of P. stellatum exhibits significant central and peripheral anti-nociceptive activity, supporting its traditional use for pain management. The presence of multiple bioactive phytochemicals further substantiates its analgesic potential.
AIM OF THE STUDY: To investigate the anti-nociceptive effects of 80 % methanol leaf extract of P. stellatum in mice.
MATERIALS AND METHODS: The central and peripheral analgesic effects of the extract were assessed at doses of 100, 200, and 400 mg/kg using the hot plate (HPT) and formalin tests (FT) in mice. Phytochemical screening was also performed, including a qualitative evaluation of 14 compounds and the quantification of total content of alkaloids, flavonoids, and phenols. Additionally, an acute toxicity test was performed to assess the safety of the plant extract. Data were analyzed using a one-way analysis of variance (ANOVA) followed by Dunnett's t-test, with statistical significance set at p < 0.05.
RESULTS: The P. stellatum extract was safe at 2000 mg/kg with no observed toxicity. Phytochemical screening confirmed the presence of alkaloids, flavonoids, phenols, saponins, tannins, sterols, terpenoids, coumarins, and fats/oils. Quantitatively, the extract demonstrated measurable levels of total flavonoids, phenols, and alkaloids. Phytochemical quantification showed flavonoids 893.7 ± 10.49 μg/g, phenols 789.76 ± 55.15 μg/g, and alkaloids 222.64 ± 17.40 μg/g (mean ± SE, n = 3). In the hot plate test (HPT), all doses significantly increased pain-reaction latency (p < 0.05). In the formalin test (FT), the 200 mg/kg and 400 mg/kg doses produced significant inhibition of paw-licking behavior in the inflammatory (late) phase (p < 0.05), while morphine inhibited both phases (p < 0.05).
CONCLUSION: The methanolic leaf extract of P. stellatum exhibits significant central and peripheral anti-nociceptive activity, supporting its traditional use for pain management. The presence of multiple bioactive phytochemicals further substantiates its analgesic potential.