A developmental study of soluble L1.

L1 is a neural cell adhesion molecule expressed by neurons and it is involved in cell interactions during axon elongation and fasciculation. L1 from rat brain consists of a membrane-inserted Mr 200,000 polypeptide from which two polypeptides of Mr 180,000 and Mr 140,000 can be derived. These latter polypeptides appear both as membrane-associated and as soluble molecules. In this report, both total and soluble L1 in rat brain have been quantified by an enzyme-linked immunosorbent assay. The amount of total L1 per gram brain varies with postnatal age showing a peak value at postnatal day 7. The variation in soluble L1 coincides with the changes in total L1. Thus, soluble L1 constitutes ca 2% of total L1 at all ages investigated. The soluble Mr 140,000 and 180,000 L1 polypeptides are also present in cerebrospinal fluid. Studies of membrane L1 catabolism in cultured fetal rat brain neurons show that the half-life of membrane L1 is less than 24 hr. As a part of membrane L1 catabolism, small amounts of soluble L1 polypeptides are released to include cell surroundings.