Biochimica et biophysica acta · 2000
A lectin from an edible mushroom Pleurotus ostreatus as a food intake-suppressing substance.
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Kawagishi H, Suzuki H, Watanabe H, Nakamura H, Sekiguchi T, Murata T, Usui T, Sugiyama K, Suganuma H, Inakuma T, Ito K, Hashimoto Y, Ohnishi-Kameyama M, Nagata T
Аннотация
In an experiment in which rats were allowed free access to food and water, the rats did not eat the diet containing a mushroom Pleurotus ostreatus even if they were emaciated. A P. ostreatus lectin (POL) was isolated from the mushroom as the food intake-suppression principle. In hemagglutination inhibition assays, Me-alphaGalNAc was the most potent inhibitor among the monosaccharides tested. Among all the sugars tested, 2'-fucosyllactose (Fucalpha1-->2Galbeta1-->4Glc) was the strongest inhibitor and its inhibitory potency was five times greater than that of Me-alphaGalNAc. POL exhibited a binding ability to bovine submaxillary mucin (BSM) and asialo-BSM and the other glycoproteins were inert to the binding. The food intake-suppressing activity of POL was dependent on the dose. The diet containing 0.1% POL caused a 50% decrease in the food intake of rats against the control.
Предметные рубрики MeSH
Amino AcidsAnimalsAppetite DepressantsCationsChromatography, Ion ExchangeDietEatingElectrophoresis, Polyacrylamide GelHemagglutination TestsHot TemperatureHydrogen-Ion ConcentrationIsoelectric FocusingLectinsMaleMetalsPlant ExtractsPleurotusRatsRats, WistarSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization