Anticancer research · 2001
Correlation between mRNA levels of IL-6 and TNF alpha and progression rate in anal squamous epithelial lesions from HIV-positive men.
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Arany I, Muldrow M, Tyring S K
Аннотация
BACKGROUND: The incidence of anal high-grade intraepithelial lesions (HSILs) and progression of anal low-grade intraepithelial lesions (LSILs) to HSIL are high in HIV-positive men. Endogenous cytokines might support the pathogenesis of this progression.
MATERIALS AND METHODS: Accordingly, we determined mRNA levels of IL-6 and TNF alpha and their receptors together with viral genes (HIV-gag and HPV E7) in biopsies of anal condylomas, LSILs and HSILs from HIV-positive individuals by a semiquantitative RT-PCR method.
RESULTS: We found that HSIL significantly differs in expression of these genes from LSIL and condylomas, and the latter two lesions were virtually undistinguishable from each other. A correlation between cytokine levels and HIV as well as HPV E7 transcripts suggests that changes might be associated with each other.
CONCLUSIONS: These findings reveal important molecular events associated with progression of anal intraepithelial lesions (ASILs) in HIV-infected men.
MATERIALS AND METHODS: Accordingly, we determined mRNA levels of IL-6 and TNF alpha and their receptors together with viral genes (HIV-gag and HPV E7) in biopsies of anal condylomas, LSILs and HSILs from HIV-positive individuals by a semiquantitative RT-PCR method.
RESULTS: We found that HSIL significantly differs in expression of these genes from LSIL and condylomas, and the latter two lesions were virtually undistinguishable from each other. A correlation between cytokine levels and HIV as well as HPV E7 transcripts suggests that changes might be associated with each other.
CONCLUSIONS: These findings reveal important molecular events associated with progression of anal intraepithelial lesions (ASILs) in HIV-infected men.
Предметные рубрики MeSH
AIDS-Related Opportunistic InfectionsAnus NeoplasmsCondylomata AcuminataDisease ProgressionHIV Core Protein p24HIV SeropositivityHumansInterleukin-6Neoplasms, Squamous CellOncogene Proteins, ViralPapillomaviridaeRNA, MessengerRNA, ViralReceptors, Interleukin-6Receptors, Tumor Necrosis FactorTumor Necrosis Factor-alpha