Insights into a hole transfer mechanism between glucose oxidase and a p-type organic semiconductor.

This manuscript describes a bioelectrochemical application of a new class of electrochemically generated hole-transporting (p-type) polymeric semiconductors (HTPS), which are based on a carbazole core and the oxiran and thiiran reactive groups. Electrode based on transparent layer of indium tin oxide was electrochemically modified with a layer of HTPS and a monolayer of covalently immobilized glucose oxidase (GOx). The HTPS/GOx-based electrode was investigated for an evaluation of direct hole-transfer between the enzyme and electrode at a bio-electrochemically relevant potential via HTPS layer. The broad linear relationship between the peak-current density and glucose concentration from 2 to 15mM and high stability of ITO/poly-CzS/GOx-electrode was observed. Moreover, it was determined that charge transfer rate constants are reliable for the establishment of advanced electron transfer between enzyme and electrode for the application of this HTPS/GOx-based electrode in long-lived biofuel cells and amperometric biosensors.