Pear Polysaccharide Alleviates Asthma Symptoms in Association With a Ferroptosis-Related Biomarker Network: An Integrated Bioinformatics and Experimental Study.

Asthma remains a prevalent global health challenge, necessitating novel therapeutic targets. This study hypothesized that pear polysaccharide alleviates asthma by modulating ferroptosis-related biomarkers. We integrated transcriptomic data (GSE143303, GSE147878) with database mining (CTD, OMIM, GeneCards, HERB, and FerrDb) to identify genes shared among asthma, pear, and ferroptosis. Machine learning screened core biomarkers, validated in independent datasets (GSE43696 and GSE63142). An ovalbumin-induced asthma mouse model was used to test the effect of Korla fragrant pear polysaccharide (10 and 40 mg/kg). Bioinformatics analysis identified 146 asthma-pear shared genes enriched in oxidative stress. Seven biomarkers (AR, CDKN1A, HMOX1, IL1B, IL6, PARP1, and TP53) were screened. In vivo, pear polysaccharide significantly attenuated cytokine release, and reversed the expression of the seven biomarkers in lung tissue and BALF of murine asthma model.Our study demonstrates that pear polysaccharide alleviates asthma pathology, potentially by targeting a hub gene network centered on ferroptosis-related biomarkers.