Dissolved organic carbon modulates the effects of copper on olfactory-mediated behaviors of chinook salmon.

The modulation of Cu effects on olfactory-mediated behaviors by dissolved organic carbon (DOC) and Fe was examined in juvenile chinook salmon. Chinook were exposed to several concentrations of Cu, DOC, and Fe alone or in combination in a flow-through system for either 4 d (acute exposure) or 14 d (subchronic exposure) and tested for their ability to detect and avoid the odorant L-histidine in an avoidance/preference trough assay. In both acute and subchronic exposures, Cu inhibited the ability of fish to detect this amino acid in a concentration-dependent manner, and Cu toxicity (olfactory inhibition) decreased with increasing DOC concentration. In both acute and subchronic experiments including DOC, Cu-induced olfactory inhibition decreased in a linear fashion with increasing DOC concentration, although the modulation was lower in subchronic exposures. The protective effect of DOC on Cu olfactory inhibition was reduced only slightly in the presence of Fe, indicating that other metals can potentially affect the modulation of the olfactory inhibition of Cu through competition for DOC binding sites. The results of the present study clearly show the amelioration by DOC of the effects of Cu on juvenile chinook salmon olfaction at a behavioral level. These data further indicate that DOC concentrations should be considered when evaluating the potential impact of Cu on fish olfaction.