Nocardioides segetis sp. nov., isolated from the sandy soil of a long-term continuous cropping cotton field.

A Gram-stain positive, aerobic, rod-shaped bacterium, designated BYT-33-1T, was isolated from a typical sandy soil in Xinjiang, PR China. This isolate grew well at 20-40 °C, pH 6.0-10.0 and 0-3.0% (w/v) NaCl, with optimal growth at 30 °C, pH 7.0 and 0.5% NaCl, respectively. Phylogenetic analysis of 16S rRNA gene sequences indicated that BYT-33-1T shared the highest sequence similarities with Nocardioides nitrophenolicus NSP 41T (98.4%) and Nocardioides kongjuensis A2-4T (98.3%). The draft genome was 5,327,936 bp, consisting of 24 contigs, with a G+C content of 72.4 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain BYT-33-1T and its closely related type strains of genus Nocardioides were 88.1-80.5% and 35.0-23.5%, respectively. The predominant menaquinone was MK-8(H4), and C18:0 10-methyl and iso-C16:0 constituted the major cellular fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, one unidentified aminolipid (AL) and three unidentified phospholipids. The ll-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. In addition, strain BYT-33-1T exhibits hexadecane degradation ability, suggesting its potential application value in alkane bioremediation of contaminated environments. Based on chemotaxonomic, phylogenetic, phenotypic and genomic analyses, we propose a novel species, named Nocardioides segetis sp. nov., with the type strain BYT-33-1T (=CCTCC AA 2024001T=KCTC 59242T).