eLife · 2024
Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.
СтатьяНазвание и аннотация: Перевод в очереди
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Lin Ya-Hui, Wang Li-Wen, Chen Yen-Hui, Chan Yi-Chieh, Hu Shang-Hsiu, Wu Sheng-Yan, Chiang Chi-Shiun, Huang Guan-Jie, Yang Shang-Da, Chu Shi-Wei, Wang Kuo-Chuan, Lin Chin-Hsien, Huang Pei-Hsin, Cheng Hwai-Jong, Chen Bi-Chang, Chu Li-An
Аннотация
Tissue-clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. HIF-Clear with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain and is able to be used as the evaluation of disease treatment efficiency. HIF-Clear also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen, as well as a 3-month formalin-fixed mouse brain. Thus, HIF-Clear represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.