Neuron · 2020
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Kodani Andrew, Kenny Connor, Lai Abbe, Gonzalez Dilenny M, Stronge Edward, Sejourne Gabrielle M, Isacco Laura, Partlow Jennifer N, O'Donnell Anne, McWalter Kirsty, Byrne Alicia B, Barkovich A James, Yang Edward, Hill R Sean, Gawlinski Pawel, Wiszniewski Wojciech, Cohen Julie S, Fatemi S Ali, Baranano Kristin W, Sahin Mustafa, Vossler David G, Yuskaitis Christopher J, Walsh Christopher A
Аннотация
Genes mutated in human neuronal migration disorders encode tubulin proteins and a variety of tubulin-binding and -regulating proteins, but it is very poorly understood how these proteins function together to coordinate migration. Additionally, the way in which regional differences in neocortical migration are controlled is completely unknown. Here we describe a new syndrome with remarkably region-specific effects on neuronal migration in the posterior cortex, reflecting de novo variants in CEP85L. We show that CEP85L is required cell autonomously in vivo and in vitro for migration, that it localizes to the maternal centriole, and that it forms a complex with many other proteins required for migration, including CDK5, LIS1, NDE1, KIF2A, and DYNC1H1. Loss of CEP85L disrupts CDK5 localization and activation, leading to centrosome disorganization and disrupted microtubule cytoskeleton organization. Together, our findings suggest that CEP85L highlights a complex that controls CDK5 activity to promote neuronal migration.