The Journal of investigative dermatology · 2025
Tick Extracellular Vesicles Alter Epidermal Keratinocyte Function.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Marnin Liron, Valencia Luisa M, Bogale Haikel N, Laukaitis-Yousey Hanna J, Rolandelli Agustin, Ferraz Camila Rodrigues, O'Neal Anya J, Schmitter-Sánchez Axel D, Cuevas Emily Bencosme, Nguyen Thu-Thuy, Leal-Galvan Brenda, Rickert David M, Mendes M Tays, Samaddar Sourabh, Butler L Rainer, Singh Nisha, Cabrera Paz Francy E, Wu-Chuang Alejandra, Oliver Jonathan D, Jameson Julie M, Munderloh Ulrike G, Oliva Chávez Adela S, Mulenga Albert, Park Sangbum, Serre David, Pedra Joao H F
Аннотация
Wound healing has been extensively studied through the lens of inflammatory disorders and cancer, but limited attention has been given to hematophagy and arthropod-borne diseases. Hematophagous ectoparasites, including ticks, subvert the wound healing response to maintain prolonged attachment and facilitate blood feeding. In this study, we unveil a strategy through which extracellular vesicles ensure blood feeding and arthropod survival in 3 medically relevant tick species. Through single-cell RNA sequencing and murine genetics, we demonstrate that wild-type animals infested with Ixodes scapularis that produce fewer extracellular vesicles display a unique epidermal subpopulation with a mesenchymal-like transcriptional program and an overrepresentation of pathways connected to wound healing. Furthermore, tick extracellular vesicles inhibit proliferation and diminish the capacity of gap closure in keratinocytes using an in vitro scratch assay. This occurrence was linked to phosphoinositide 3-kinase activity, keratinocyte GF 1, and TGF-β levels. Collectively, we uncovered a strategy employed by a blood-feeding arthropod that disrupts the circuitry in cutaneous wound healing, contributing to ectoparasite fitness.