Acta tropica · 2025
Description and characterization of Borrelia genospecies in infected patients in the Western region of Venezuela.
СтатьяНазвание и аннотация: Перевод в очереди
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Rivas José Goyo, Rendon Mariolly, Maldonado Solsiree, Abraham Lousdy Carolina Gómez, Andará Darling Stella Morales, Dávila-Vera Delsy, Mendoza-Briceño Rosa Virginia, Salmen Siham
Аннотация
BACKGROUND: Lyme disease, or Lyme borreliosis (LB), is the most prevalent vector-borne disease, representing over 75 % of tick-borne illnesses, with approximately 700,000 cases reported annually in the United States and Europe. No studies in Venezuela have explored the presence or genospecies diversity of Borrelia spp. This study aimed to detect and characterize Borrelia spp. circulating in Venezuela.
METHODS: A cohort of 1140 patients was analyzed using microscopy, real-time PCR, enzyme immunoassay (EIA), and Western blot to identify Borrelia spp. and associated hemoparasites.
RESULTS: Borrelia spp. were detected in 188 (16.49 %) patients, with 130 (69.14 %) exhibiting coinfections with other hemoparasites, such as Babesia spp. and Ehrlichia spp. The predominant genospecies was B. garinii (43.5 % IgM+, 13 % IgG+), followed by B. spielmanii (21.7 % IgM+, 4.3 % IgG+), B. afzelii (19.6 % IgM+, 2.2 % IgG+), B. burgdorferi sensu stricto (Bbss) (10.9 % IgM+, 6.5 % IgG+), and B. bavariensis (2.2 % IgM+, 4.3 % IgG+). Co-occurrence of multiple genospecies was observed in 36.73 % of cases.
CONCLUSIONS: This study in Venezuela confirms the presence of Lyme borreliosis and highlights the diversity of circulating genospecies. The use of sensitive, broad-spectrum diagnostic tools is critical to reducing false negatives and ensuring timely diagnosis, thereby improving patient outcomes.
METHODS: A cohort of 1140 patients was analyzed using microscopy, real-time PCR, enzyme immunoassay (EIA), and Western blot to identify Borrelia spp. and associated hemoparasites.
RESULTS: Borrelia spp. were detected in 188 (16.49 %) patients, with 130 (69.14 %) exhibiting coinfections with other hemoparasites, such as Babesia spp. and Ehrlichia spp. The predominant genospecies was B. garinii (43.5 % IgM+, 13 % IgG+), followed by B. spielmanii (21.7 % IgM+, 4.3 % IgG+), B. afzelii (19.6 % IgM+, 2.2 % IgG+), B. burgdorferi sensu stricto (Bbss) (10.9 % IgM+, 6.5 % IgG+), and B. bavariensis (2.2 % IgM+, 4.3 % IgG+). Co-occurrence of multiple genospecies was observed in 36.73 % of cases.
CONCLUSIONS: This study in Venezuela confirms the presence of Lyme borreliosis and highlights the diversity of circulating genospecies. The use of sensitive, broad-spectrum diagnostic tools is critical to reducing false negatives and ensuring timely diagnosis, thereby improving patient outcomes.