Eleven previously undescribed sesquiterpenoids with anti-inflammatory activity isolated from the cultures of basidiomycete Marasmiellus candidus.

In this study, eleven undescribed sesquiterpenoids, named marasmiellolides A-K (1-11), were isolated from the rice-fermented mycelia of the basidiomycete Marasmiellus candidus. Their structures were established through extensive spectroscopic analyses including HR-ESI-MS, 1D and 2D NMR. The absolute configurations of compounds 1-3 and 5-8 were assigned based on the comparison of the experimental and calculated ECD data. In addition, compounds 1-11 were tested for anti-inflammatory activity against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages. Compounds 1-3 exhibited significant anti-inflammatory activity with IC50 values ranging from 7.2 to 13.2 μM.