Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2004
Analysis of TGF-beta2 and TGF-beta3 expression in the hydrocephalic H-Tx rat brain.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Li Xianfeng, Miyajima Masakazu, Arai Hajime
Аннотация
INTRODUCTION: Transforming growth factor-beta (TGF-beta) is an important cytokine with modulatory actions in the nervous system. The development of hydrocephalus in mouse models resulting from the overexpression of TGF-beta1 has previously been described, but the mechanism by which this occurs remains obscure.
METHODS: In order to evaluate the role of TGF-beta in hydrocephalus, we used SYBR Green I-based real-time quantitative RT-PCR method and Western blot analysis to analyze the TGF-beta2 and TGF-beta3 mRNA and protein expressions in the cerebral cortex of the H-Tx rat, a model of congenital hydrocephalus.
RESULTS: The hydrocephalic H-Tx rat expressed significantly higher TGF-beta3 levels than their normal siblings (p<0.01) at 7 and 14 days of age. This difference became insignificant when analyzed at 21 days of age. On the other hand, such a difference has not been observed in the TGF-beta2 levels in the hydrocephalic H-Tx rat.
CONCLUSIONS: These results suggest that TGF-beta2 and TGF-beta3 expression may be modulated differently in the hydrocephalus, and TGF-beta3 may contribute to the development of hydrocephalus in this rat model.
METHODS: In order to evaluate the role of TGF-beta in hydrocephalus, we used SYBR Green I-based real-time quantitative RT-PCR method and Western blot analysis to analyze the TGF-beta2 and TGF-beta3 mRNA and protein expressions in the cerebral cortex of the H-Tx rat, a model of congenital hydrocephalus.
RESULTS: The hydrocephalic H-Tx rat expressed significantly higher TGF-beta3 levels than their normal siblings (p<0.01) at 7 and 14 days of age. This difference became insignificant when analyzed at 21 days of age. On the other hand, such a difference has not been observed in the TGF-beta2 levels in the hydrocephalic H-Tx rat.
CONCLUSIONS: These results suggest that TGF-beta2 and TGF-beta3 expression may be modulated differently in the hydrocephalus, and TGF-beta3 may contribute to the development of hydrocephalus in this rat model.
Предметные рубрики MeSH
Age FactorsAnimalsAnimals, NewbornBenzothiazolesBlotting, WesternDiaminesGene Expression Regulation, DevelopmentalHydrocephalusOrganic ChemicalsQuinolinesRNA, MessengerRatsRats, Inbred StrainsReverse Transcriptase Polymerase Chain ReactionStatistics, NonparametricTransforming Growth Factor betaTransforming Growth Factor beta2Transforming Growth Factor beta3