[Extract berberine hydrochloride by aqueous two-phase system].

OBJECTIVE

To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride.

METHODS

Three aqueous two-phase systems were used for extraction experiment firstly, the best one was chosen from that, and then the single factor experiment and orthogonal experiment were carried out.

RESULTS

10 mL 95% ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system, added 600 mg berberine hydrochloride whose purity was 53.22% into it, regulated its pH to 4, then the system was put into water-bath heater (70 degrees C) for 30 min, the extraction rate could reach 99.29%; Collected the extraction liquid, dried it under 40 degrees C, the purity of berberine hydrochloride was 88.43%.

CONCLUSION

This system is a suitable aqueous two-phase system for extraction of berberine hydrochloride.