Replacing methanol with internally produced VFA-based carbon source for denitrification at the Henriksdal WWTP.

To meet future nitrogen removal targets, Henriksdal wastewater treatment plant (WWTP) will require external carbon addition, estimated at 8 tons COD/day by 2040, due to low influent BOD/TN ratios, precipitation chemical use, and low temperatures. Methanol, the projected option, is fossil-based and contributes to indirect greenhouse gas emissions. This study evaluated a volatile fatty acid (VFA)-based fermentate produced from primary sludge and food waste in a 2 m3 mesophilic fermenter as a carbon source for denitrification. The filtrated fermentate was tested as carbon source in both batch denitrification tests and pilot-scale MBR (4.5 m3/h), where it was dosed for 70 days, replacing glycerol currently used at Henriksdal and Henriksdals WWTP. In batch tests, the fermentate achieved a 40% higher denitrification rate than glycerol. Pilot trials showed a 30% higher denitrification rate and 50% lower carbon consumption while maintaining effluent concentration below 3 mg NO3- -N/L. Microbial analysis revealed no significant community changes with the carbon source transition, indicating effective VFA uptake by existing microorganisms. Full-scale projections suggested that replacing methanol would require 10% of the plant's primary sludge plus food waste. Although this sludge use would reduce biogas production, methane potential tests showed that recycling of the fermentate solid fraction would result in only 2% lower biogas production, representing a minor trade-off.