PloS one · 2011
Venous graft-derived cells participate in peripheral nerve regeneration.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАПоддержка правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Lavasani Mitra, Gehrmann Sebastian, Gharaibeh Burhan, Clark Katherine A, Kaufmann Robert A, Péault Bruno, Goitz Robert J, Huard Johnny
Аннотация
BACKGROUND: Based on growing evidence that some adult multipotent cells necessary for tissue regeneration reside in the walls of blood vessels and the clinical success of vein wrapping for functional repair of nerve damage, we hypothesized that the repair of nerves via vein wrapping is mediated by cells migrating from the implanted venous grafts into the nerve bundle.
METHODOLOGY/PRINCIPAL FINDINGS: To test the hypothesis, severed femoral nerves of rats were grafted with venous grafts from animals of the opposite sex. Nerve regeneration was impaired when decellularized or irradiated venous grafts were used in comparison to untreated grafts, supporting the involvement of venous graft-derived cells in peripheral nerve repair. Donor cells bearing Y chromosomes integrated into the area of the host injured nerve and participated in remyelination and nerve regeneration. The regenerated nerve exhibited proper axonal myelination, and expressed neuronal and glial cell markers.
CONCLUSIONS/SIGNIFICANCE: These novel findings identify the mechanism by which vein wrapping promotes nerve regeneration.
METHODOLOGY/PRINCIPAL FINDINGS: To test the hypothesis, severed femoral nerves of rats were grafted with venous grafts from animals of the opposite sex. Nerve regeneration was impaired when decellularized or irradiated venous grafts were used in comparison to untreated grafts, supporting the involvement of venous graft-derived cells in peripheral nerve repair. Donor cells bearing Y chromosomes integrated into the area of the host injured nerve and participated in remyelination and nerve regeneration. The regenerated nerve exhibited proper axonal myelination, and expressed neuronal and glial cell markers.
CONCLUSIONS/SIGNIFICANCE: These novel findings identify the mechanism by which vein wrapping promotes nerve regeneration.
Предметные рубрики MeSH
AnimalsAxonsCell MovementCell ProliferationCells, CulturedFemaleFemoral NerveFemoral VeinImmunohistochemistryIn Situ Hybridization, FluorescenceMaleMicroscopy, Electron, TransmissionNerve Fibers, MyelinatedNerve RegenerationNeurofilament ProteinsRatsRats, Inbred F344Schwann CellsSex FactorsTransplantation, Homologous