Nephrology (Carlton, Vic.) · 2012
Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease.
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Abbiss Hayley, Maker Garth L, Gummer Joel, Sharman Matthew J, Phillips Jacqueline K, Boyce Mary, Trengove Robert D
Аннотация
AIM: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat.
METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups.
RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model.
CONCLUSION: The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.
METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups.
RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model.
CONCLUSION: The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.