A Direct Dilute-and-Shoot HPLC-MS/MS Method for Tetrahydrocannabinol (THC) Metabolites.

The Cannabis plant has been smoked for medicinal and recreational purposes for thousands of years. Tetrahydrocannabinol (THC) is the most well-known psychoactive cannabinoid, and the properties of other cannabinoids are becoming better understood. Due to increased exposure, hospitals and clinics will need access to rapid and accurate THC testing procedures to better inform patients and improve care. A rapid and reliable HPLC-MS/MS method was developed for the quantitative assessment of two THC metabolites (THC-COOH and THC-COO(Gluc)). The chromatographic separation was done using a short (50 × 4.6 mm) phenyl-hexyl column with positive ESI mass spectrometry analysis. To reduce interferences and improve quantitation, the assay was run using multiple reaction monitoring mode. The method was shown to be precise (<15% CV with-in day & between day), accurate (±20%) and linear (>R2 0.99) within the range of 25-8000 ng/mL.