Cancer genomics & proteomics · 2018
The Dual PI3K/mToR Inhibitor Omipalisib/GSK2126458 Inhibits Clonogenic Growth in Oncogenically-transformed Cells from Neurocutaneous Melanocytosis.
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Basu Dipanjan, Salgado Cláudia M, Bauer Bruce, Khakoo Yasmin, Patel Janki R, Hoehl Ryan M, Bertolini Dominique M, Zabec Joie, Brzozowski Morgan R, Reyes-Múgica Miguel
Аннотация
BACKGROUND: Omipalisib has been found to affect the viability of cancer cells. However, its effect on clonogenicity - a feature of cancer stem cells, is not clear. Cells isolated from neurocutaneous melanocytosis (NCM) patients' lesions grow clonogenically. The aim of this study was to investigate the effect of omipalisib treatment on clonogenic growth of NCM cells in vitro.
MATERIALS AND METHODS: Clonogenic growth efficiency was evaluated by colony formation assays with or without specific growth factors. Activation of MEK and Akt was determined by immunoblots. Colony formation and cell viability were assessed upon pharmacological inhibition of MEK, Akt and mToR.
RESULTS: Clonogenicity appeared to depend on bFGF and IGF1signaling through ERK and Akt. Omipalisib treatment prevented colony formation and induced autophagic cell death.
CONCLUSION: Signaling through Akt is important for survival of clonogenic cells in NCM, and omipalisib treatment as a monotherapy or in combination with MEK162 could be an effective therapeutic strategy to inhibit clonogenic growth.
MATERIALS AND METHODS: Clonogenic growth efficiency was evaluated by colony formation assays with or without specific growth factors. Activation of MEK and Akt was determined by immunoblots. Colony formation and cell viability were assessed upon pharmacological inhibition of MEK, Akt and mToR.
RESULTS: Clonogenicity appeared to depend on bFGF and IGF1signaling through ERK and Akt. Omipalisib treatment prevented colony formation and induced autophagic cell death.
CONCLUSION: Signaling through Akt is important for survival of clonogenic cells in NCM, and omipalisib treatment as a monotherapy or in combination with MEK162 could be an effective therapeutic strategy to inhibit clonogenic growth.
Предметные рубрики MeSH
ApoptosisCell ProliferationCell Transformation, NeoplasticFemaleHumansInfantMelanomaMelanosisNeurocutaneous SyndromesPhosphoinositide-3 Kinase InhibitorsProto-Oncogene Proteins c-aktPyridazinesQuinolinesSignal TransductionSkin NeoplasmsSulfonamidesTOR Serine-Threonine KinasesTumor Cells, CulturedTumor Stem Cell AssayBenzenesulfonamides