Development of a H2S-responsive NIR Fluorescent Probe for H2S Detection and H2S Releasing Monitoring From Prodrug.

H2S was deemed as a toxic gradient in the realm of food and environment but plays pivotal pathophysiological roles in organisms. H2S instabilities and disturbances are always responsible for multiple disorders. We fabricated a H2S-responsive NIR fluorescent probe (HT) for H2S detection and evaluation both in vitro and in vivo. HT exhibited rapid H2S response within 5 min, accompanied with visible color change and NIR fluorescence generation, and the fluorescent intensities were linearly correlated with corresponding H2S concentrations. When HT was incubated with A549 cells, the intracellular H2S and H2S fluctuations could be monitored ore rotundo via the responsive fluorescence. Meanwhile, when HT was co-administrated with H2S prodrug ADT-OH, the H2S release from ADT-OH could be visualized and monitored to evaluate its release efficacy.