Immunity · 2020
A Potent Anti-Malarial Human Monoclonal Antibody Targets Circumsporozoite Protein Minor Repeats and Neutralizes Sporozoites in the Liver.
Клиническое исследование I фазыСтатьяПоддержка NIH: внешняя программаResearch Support, N.I.H., IntramuralПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Wang Lawrence T, Pereira Lais S, Flores-Garcia Yevel, O'Connor James, Flynn Barbara J, Schön Arne, Hurlburt Nicholas K, Dillon Marlon, Yang Annie S P, Fabra-García Amanda, Idris Azza H, Mayer Bryan T, Gerber Monica W, Gottardo Raphael, Mason Rosemarie D, Cavett Nicole, Ballard Reid B, Kisalu Neville K, Molina-Cruz Alvaro, Nelson Jorgen, Vistein Rachel, Barillas-Mury Carolina, Amino Rogerio, Baker David, King Neil P, Sauerwein Robert W, Pancera Marie, Cockburn Ian A, Zavala Fidel, Francica Joseph R, Seder Robert A
Аннотация
Discovering potent human monoclonal antibodies (mAbs) targeting the Plasmodium falciparum circumsporozoite protein (PfCSP) on sporozoites (SPZ) and elucidating their mechanisms of neutralization will facilitate translation for passive prophylaxis and aid next-generation vaccine development. Here, we isolated a neutralizing human mAb, L9 that preferentially bound NVDP minor repeats of PfCSP with high affinity while cross-reacting with NANP major repeats. L9 was more potent than six published neutralizing human PfCSP mAbs at mediating protection against mosquito bite challenge in mice. Isothermal titration calorimetry and multiphoton microscopy showed that L9 and the other most protective mAbs bound PfCSP with two binding events and mediated protection by killing SPZ in the liver and by preventing their egress from sinusoids and traversal of hepatocytes. This study defines the subdominant PfCSP minor repeats as neutralizing epitopes, identifies an in vitro biophysical correlate of SPZ neutralization, and demonstrates that the liver is an important site for antibodies to prevent malaria.