Fertility and sterility · 2015
Presence of human papillomavirus in semen of healthy men is firmly associated with HPV infections of the penile epithelium.
СтатьяНаблюдательное исследованиеНазвание и аннотация: Перевод в очереди
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Luttmer Roosmarijn, Dijkstra Maaike G, Snijders Peter J F, Jordanova Ekaterina S, King Audrey J, Pronk Divera T M, Foresta Carlo, Garolla Andrea, Hompes Peter G A, Berkhof Johannes, Bleeker Maaike C G, Doorbar John, Heideman Daniëlle A M, Meijer Chris J L M
Аннотация
OBJECTIVE: To study the source of human papillomavirus (HPV) in semen.
DESIGN: Observational study (CCMO-NL3248800010).
SETTING: Academic hospital-based laboratory.
PATIENT(S): Healthy male volunteers (n = 213).
INTERVENTION(S): One penile scrape and three semen samples were obtained per participant for HPV-DNA testing by both GP5+/6+ polymerase chain reaction (PCR) and SPF10-PCR to detect moderate/high and low viral loads, respectively; flat penile lesions (FPL) were detected by penoscopy.
MAIN OUTCOME MEASURE(S): HPV-DNA presence in semen and penile scrapes, and the presence of FPL.
RESULT(S): HPV-DNA at moderate/high viral loads (i.e., GP5+/6+ PCR-positive) was detected in ≥1 semen sample(s) in 27% of participants. Most men with moderate/high viral loads in the penile scrape also had moderate/high viral loads in semen (85%). Men with a HPV-negative penile scrape were very unlikely to have moderate/high viral loads in semen (3%). The presence of HPV in semen was associated with the presence of HPV in the penile scrape also on a genotype-specific level. Having FPL was a risk factor for HPV presence in semen.
CONCLUSION(S): HPV-DNA presence in semen of healthy men is common and associated with HPV infections of the penile epithelium. HPV-DNA presence in semen may result from desquamation of HPV-infected penile cells.
DESIGN: Observational study (CCMO-NL3248800010).
SETTING: Academic hospital-based laboratory.
PATIENT(S): Healthy male volunteers (n = 213).
INTERVENTION(S): One penile scrape and three semen samples were obtained per participant for HPV-DNA testing by both GP5+/6+ polymerase chain reaction (PCR) and SPF10-PCR to detect moderate/high and low viral loads, respectively; flat penile lesions (FPL) were detected by penoscopy.
MAIN OUTCOME MEASURE(S): HPV-DNA presence in semen and penile scrapes, and the presence of FPL.
RESULT(S): HPV-DNA at moderate/high viral loads (i.e., GP5+/6+ PCR-positive) was detected in ≥1 semen sample(s) in 27% of participants. Most men with moderate/high viral loads in the penile scrape also had moderate/high viral loads in semen (85%). Men with a HPV-negative penile scrape were very unlikely to have moderate/high viral loads in semen (3%). The presence of HPV in semen was associated with the presence of HPV in the penile scrape also on a genotype-specific level. Having FPL was a risk factor for HPV presence in semen.
CONCLUSION(S): HPV-DNA presence in semen of healthy men is common and associated with HPV infections of the penile epithelium. HPV-DNA presence in semen may result from desquamation of HPV-infected penile cells.