[Interaction of alpha-latrotoxin with synaptosomes; metabolic control of the induction of calcium channels].

The aim of the present study is to elucidate the possible significance of metabolic control of the calcium permeability of synaptosomes induced by alpha-latrotoxin. The alpha-latrotoxin influence of processes of GABA release and reuptake is shown to depend on synaptosomal metabolism. It has been found that incubation by the synaptosomes during one hour at 37 degrees C or their treatment by 10(-3) M monoiodacetate during 10 min at 10 degrees C or at room temperature completely abolished or at least strongly decreased the ability of alpha-latrotoxin to induce the calcium influx. Under the same conditions the ability of alpha-latrotoxin to activate the GABA release and inhibit the GABA reuptake do not change. It is concluded that only formation of calcium channels by alpha-latrotoxin is strictly controlled by the level of synaptosomal metabolism.