Isolation and characterization of the specific peptides from rabbit blastocyst at peri-implantation stage with emphasis on their physiological significance relevant to the mechanism of implantation.

The secretory materials from the uterine flushings of the pregnant, non-pregnant and pseudopregnant rabbits were analysed by the method of SDS-polyacrylamide gel electrophoresis. The results demonstrated that there are three specific peptides in the pregnant uterine flushings with molecular weights of 3000, 4500 and 6000. No correspondent peptides were found in non-pregnant and pseudopregnant uterine flushings. The peptides were also isolated and identified from the blastocystic fluid. At least 4 peaks of small peptides were shown by high pressure liquid chromatography. The range of their isoelectrophoretic points is between pH 4.0-5.5 Concavalin A (Con A) affinitive electrophoresis indicated that they are glycopeptides in nature. The LH-RH A is able to exert inhibitory effects on the synthesis of these peptides. Their possible physiological function has been discussed.