Multi-level structured nanoparticles prepared by microfluidics control method for efficient and sensitive chemiluminescence immunoassay.

The properties of nanomaterials are heavily influenced by their size effects. Utilizing the self-assembly principle offers a promising avenue for crafting innovative nanomaterials, yet controlling this process at the microscopic level presents significant challenges, hindering effective regulation of nanomaterial morphology. Microfluidic technology, however, offers precise control over fluid velocities within microchannels, enabling micro-level manipulation. In this study, we synthesized amphiphilic molecules HP (Hematin@NH2-PEG-COOH) through hematin modification, leveraging microfluidic techniques to encapsulate luminol within HP molecules, resulting in the formation of highly efficient chemiluminescence (CL) HPL (HP@Luminol) nanoparticles. The size effects and intricate multi-level structures achieved during encapsulation endowed these nanoparticles with enhanced catalytic capabilities for CL. Finally, we used HPL nanoparticles as luminescent markers to develop a CL immunosensor for the sensitive detection of the tumor marker carcinoembryonic antigen (CEA), achieving satisfactory results. This innovative approach not only expands the repertoire of nanomaterial design and synthesis but also offers a practical solution for sensitive biomarker detection. Overall, this research introduces a novel strategy for designing and fabricating advanced nanomaterials, underscoring the potential of microfluidic technology in nanoscience and biosensing applications.