β2 adrenergic receptors and leptin interplay to decrease food intake in chicken.

1. The present study was designed to examine the effects of intracerebroventricular (ICV) injection of different α and [Formula: see text] adrenergic receptor antagonists on leptin-induced hypophagia in broiler chickens.2. The study consisted of six experiments. In all experiments, chickens were deprived of feed for 3 h prior to the ICV injections and thereafter were returned immediately to the individual cages and cumulative feed intake, based on the percentage of body weight, was measured at 30, 60 and 120 min post-injection.3. In experiment 1, leptin (2.5, 5 or 10 µg) were injected in birds. In experiment 2, groups received either control solution, prazosin (10 nmol), leptin (10 µg) or a co-injection of prazosin (10 nmol) and leptin (10 µg). The other experiments were conducted as experiment 2, but instead of prazosine (10 nmol), yohimbine (13 nmol) was used in experiment 3, metoprolol (24 nmol) in experiment 4, ICI 118,551 (5 nmol) in experiment 5 and SR 59230R (5 nmol) in experiment 6 were injected either in a group or in combination with leptin (10 µg).4. The results of this study revealed a dose-dependent hypophagic effect of leptin and, in experiment 5, ICV co-injection of ICI118, 551 (5 nmol) and leptin (10 µg) significantly attenuated this effect (P˂0.5). These results suggest that the hypophagic effect of leptin is probably mediated by β2 adrenergic receptors in chickens.