Laboratory Conversion of Cultivated Oleaginous Organisms into Biocrude for Biofuel Applications.

Hydrothermal liquefaction (HTL) is a thermochemical process for the wet conversion of oleaginous microorganisms and other biomass and carbon rich feedstocks to biofuels under subcritical conditions. It is a novel green process that produces biocrude as a primary product along with other by-products which include gases, aqueous phase coproduct (ACP) and solid residues. Here we describe in detail the protocols for the conversion of biomass to biocrude through HTL and separation, quantification and analyses of HTL products.