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Войти в исследования Birth defects research. Part A, Clinical and molecular teratology · 2009
Ljungan virus present in intrauterine fetal death diagnosed by both immunohistochemistry and PCR.
Клинический случайСтатьяНазвание и аннотация: Перевод в очереди
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Samsioe Annika, Papadogiannakis Nikos, Hultman Thomas, Sjöholm Ake, Klitz William, Niklasson Bo
Аннотация
OBJECTIVES: Following up on prior evidence from animal and human studies of Ljungan virus (LV) in intrauterine fetal death (IUFD), we examine additional cases of IUFD using two standard assays of viral detection: immunohistochemistry (IHC) and real time RT-PCR.
MATERIALS AND METHODS: Frozen and formalin-fixed specimens from IUFD cases were tested for the presence of LV using real time RT-PCR and IHC, respectively. Formalin-fixed organs from terminated pregnancies diagnosed as trisomy 21 were used as controls in the IHC assay.
RESULTS: Presence of LV was demonstrated in all five IUFD cases by IHC and further confirmed in three of these cases by real time RT-PCR. Only one of 18 trisomy 21 controls was LV positive by IHC.
CONCLUSION: The presence of LV in IUFD patients has been confirmed by two different assays.
MATERIALS AND METHODS: Frozen and formalin-fixed specimens from IUFD cases were tested for the presence of LV using real time RT-PCR and IHC, respectively. Formalin-fixed organs from terminated pregnancies diagnosed as trisomy 21 were used as controls in the IHC assay.
RESULTS: Presence of LV was demonstrated in all five IUFD cases by IHC and further confirmed in three of these cases by real time RT-PCR. Only one of 18 trisomy 21 controls was LV positive by IHC.
CONCLUSION: The presence of LV in IUFD patients has been confirmed by two different assays.