Resveratrol Normalizes the Deterioration of Smooth Muscle Contractility after Intestinal Ischemia and Reperfusion in Rats Associated With an Antioxidative Effect and Modulating Tumor Necrosis Factor Alpha Activity.

BACKGROUND

The aim of the present study was to investigate the effect of resveratrol on cytokine levels and oxidative stress in intestinal ischemia/reperfusion (I/R) injuries.

METHODS

To induce intestinal I/R, the superior mesenteric artery was occluded for 30 min and then reperfused for 150 min or 24 h. The therapeutic effects of resveratrol on the damage from intestinal I/R were investigated using an isolated organ bath, along with oxidant/antioxidant and inflammatory factors such as glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-(IL)-1β, and tumor necrosis factor alpha (TNF-α).

RESULTS

I/R control animals demonstrated severe deterioration of smooth muscle motor function as a significant decrease in potassium chloride- and acetylcholine-induced a contractile responses; high oxidative stress as an increase in lipid peroxidation and a decrease in GSH level; and an increase of MPO, IL-1β, and TNF-α activity. Pretreatment of animals with resveratrol restored intestinal dysfunction; reduced elevated MDA, MPO, IL-1β, and TNF-α levels; and reversed the depleted intestine GSH levels after both 150 min and 24 h reperfusion periods.

CONCLUSIONS

The results indicated that resveratrol can reverse the effects of disrupted smooth muscle contractility, probably because of its antioxidant and anti-inflammatory effects on MPO, IL-1β, and TNF-α activities.