Diterpenoid alkaloids from Aconitum gymnandrum Maxim. and their anti-inflammatory activity.

Research on the aerial parts of Aconitum gymnandrum Maxim. led to identifying three undescribed diterpenoid alkaloids gymnaconitines H-J (1-3), along with eleven known analogues (4-14). Based on 1D/2D NMR and HRESIMS, the structures of these compounds were successfully determined. Additionally, the inhibitory effects of all compounds on NO production were evaluated in lipopolysaccharide-induced RAW264.7 cells. It is noteworthy that compounds 1, 3 and 6 demonstrated significant inhibitory effects, showing IC50 values of 10.36 ± 1.02 μM, 12.87 ± 1.11 μM, and 19.67 ± 1.29 μM, respectively. Using target screening and molecular docking technology, it is hypothesized that compound 1 may play an anti-inflammatory role by combining with Flamin-B.