Scientific reports · 2018
Bat-mouse bone marrow chimera: a novel animal model for dissecting the uniqueness of the bat immune system.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Yong Kylie Su Mei, Ng Justin Han Jia, Her Zhisheng, Hey Ying Ying, Tan Sue Yee, Tan Wilson Wei Sheng, Irac Sergio Erdal, Liu Min, Chan Xue Ying, Gunawan Merry, Foo Randy Jee Hiang, Low Dolyce Hong Wen, Mendenhall Ian Hewitt, Chionh Yok Teng, Dutertre Charles-Antoine, Chen Qingfeng, Wang Lin-Fa
Аннотация
Bats are an important animal model with long lifespans, low incidences of tumorigenesis and an ability to asymptomatically harbour pathogens. Currently, in vivo studies of bats are hampered due to their low reproduction rates. To overcome this, we transplanted bat cells from bone marrow (BM) and spleen into an immunodeficient mouse strain NOD-scid IL-2R-/- (NSG), and have successfully established stable, long-term reconstitution of bat immune cells in mice (bat-mice). Immune functionality of our bat-mouse model was demonstrated through generation of antigen-specific antibody response by bat cells following immunization. Post-engraftment of total bat BM cells and splenocytes, bat immune cells survived, expanded and repopulated the mouse without any observable clinical abnormalities. Utilizing bat's remarkable immunological functions, this novel model has a potential to be transformed into a powerful platform for basic and translational research.