Effects of steroid hormones on pituitary immunoreactive gonadotropin in European freshwater eel, Anguilla anguilla L.

The low pituitary immunoreactive gonadotropin (IR GTH) contents of immature female or male freshwater eels were strongly increased after intraperitoneal injections of estradiol. Using female eels, the effect was shown to be dose dependent. In neither sex was modification of pituitary IR GTH observed after progesterone treatment; a very small increase (significant in females but not in males) was produced by cortisol. An important sexual difference occurred with testosterone, which strongly stimulated pituitary IR GTH level in male but not in female eels. The possibility of a low central aromatase activity responsible for the lack of effect of testosterone in the female eel is discussed. Circulating IR GTH levels were very low in steroid-treated eels, as in controls, indicating a weak GTH release. Thus, as in juvenile, immature fish from other species, a sexual steroid stimulated eel pituitary GTH content; the peculiarity of the apparent "blockade" of eel sexual development appears not related to a lack of sensitivity of gonadotropin synthesis to the positive feedback of a sexual steroid.