Optimization of column chromatography technique for the isolation of tetrandrine from Cyclea peltata and LC-ESI-MS based quantification and validation of the method.

Reverse phase column chromatography technique was employed to isolate tetrandrine from methanolic extract of Cyclea peltata roots. Tetrandrine was efficiently isolated using 0.02% aqueous diethylamine and methanol (25:75, v/v) as mobile phase with a purity of 98.63%. Compound characterisation was achieved using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and other spectroscopic methods. In addition, quantification of tetrandrine and validation of the method was carried out following International Conference on Harmonisation guidelines. The study provides a simple and cost effective method to isolate substantially good quantity and pure tetrandrine from Cyclea peltata.