Enantiomer separation of dimethyl dicarboxy alpha-biphenyl (DDB) and its analogues on a covalently bonded cellulose tris-(3,5-dimethylphenylcarbamate) CSP.

Dimethyl dicarboxy alpha-biphenyl (DDB) and its analogues represent atropisomers which have been resolved on the covalently bonded cellulose tris-(3,5-dimethylphenylcarbamate) (CDMPC) CSP. Different kinds of alcohols, tetrahydrofuran (THF), and chloroform were employed as mobile phase modifiers (MPMs), and their influence on the retention and separation of the enantiomers was investigated. Ternary mobile phases (hexane/2-propanol/THF, hexane/2-propanol/chloroform) were employed to investigate the separation of the five enantiomers. The advantages of the broader choice of solvents offered by the covalently bonded CDMPC CSP were discussed. The effect of structural variation of the enantiomers on their retention and separation was investigated.