Arerugi = [Allergy] · 2023
[ANTIGENICITY OF SOY PROTEIN IN SOY-BASED INFANT FORMULA BONLACT® i].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Naito Michihiro, Matsui Teruaki, Iwawaki Yukiko, Ito Komei, Izumi Hidehiko
Аннотация
BACKGROUND: The purpose of this study was to compare the antigenicity of Bonlact® i (BL) with that of defatted soy protein (SP) and soy protein isolate (SPI), which is the original source of BL, using sera from patients with soybean allergy.
METHODS: Proteins were extracted from SP, SPI, and BL using PBS. Proteins in each sample were analyzed for antigenicity using inhibition ELISA with SP-specific IgE (sIgE), SDS-PAGE, and immunoblotting. Sere from patients with soybean allergy confirmed by an oral food challenge (OFC) (n=6, OFC+ Pt), and from patients who were positive for soy-sIgE without symptoms ( n = 7, sIgE+ Pt) were used for these assays. The cross-antigenicity of SP and BL with cow's milk (CM) proteins was also analyzed in the sera from patients with CM allergy using inhibition ELISA.
RESULTS: SDS-PAGE showed that the proteins in BL produced a smear-like band in the low-molecular-weight region compared with that in SP and SPI. Inhibition ELISA against SP-sIgE showed that BL had a significantly lower inhibition rate than that of SP in both OFC+ Pt and sIgE+ Pt. Immunoblotting analysis showed that the bands of BL were thinner than those of SP and SPI. Additionally, SP and BL showed no cross-antigenicity with CM proteins.
CONCLUSION: The proteins in BL was partially digested, and its antigenicity was lower than that of SP and SPI.
METHODS: Proteins were extracted from SP, SPI, and BL using PBS. Proteins in each sample were analyzed for antigenicity using inhibition ELISA with SP-specific IgE (sIgE), SDS-PAGE, and immunoblotting. Sere from patients with soybean allergy confirmed by an oral food challenge (OFC) (n=6, OFC+ Pt), and from patients who were positive for soy-sIgE without symptoms ( n = 7, sIgE+ Pt) were used for these assays. The cross-antigenicity of SP and BL with cow's milk (CM) proteins was also analyzed in the sera from patients with CM allergy using inhibition ELISA.
RESULTS: SDS-PAGE showed that the proteins in BL produced a smear-like band in the low-molecular-weight region compared with that in SP and SPI. Inhibition ELISA against SP-sIgE showed that BL had a significantly lower inhibition rate than that of SP in both OFC+ Pt and sIgE+ Pt. Immunoblotting analysis showed that the bands of BL were thinner than those of SP and SPI. Additionally, SP and BL showed no cross-antigenicity with CM proteins.
CONCLUSION: The proteins in BL was partially digested, and its antigenicity was lower than that of SP and SPI.