Toxic effects of naturally-aged microplastics on zebrafish juveniles: A more realistic approach to plastic pollution in freshwater ecosystems.

We aim at evaluating the toxicity of naturally-aged polystyrene microplastics (MPs) in Danio rerio at intermediate development stage. Animal models were stactically exposed to 4 × 104 and 4 × 106 microparticles/m3 for five days - this concentration is environmentally relevant. We evaluated MP's impact on animals' nutritional status and REDOX balance, as well as its potential neuro- and cytotoxic action on them. Initially, MPs did not induce any change in total carbohydrates, triglycerides and total cholesterol levels. MP accumulation was associated with oxidative stress induction, which was inferred by the nitrite and thiobarbituric acid reactive substances levels. Furthermore, we observed that such stress was not counterbalanced by increase in the assessed enzymatic (total glutathione, catalase and superoxide dismutase) and non-enzymatic (total thiols, reduced glutathione and DPPH radical scavenging activity) antioxidants. The association between high acetylcholinesterase activity and numerical changes in neuroblasts distributed on animals' body surface confirmed MP's neurotoxic potential. MP's ability to induce apoptosis and necrosis processes in animals' erythrocytes suggested its cytotoxic action; therefore, the present study is pioneer in providing insight on how MPs can affect young freshwater fish at environmental concentrations. It is essential knowing the magnitude of these pollutants' impact on the ichthyofauna.