Scientific reports · 2014
Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Fukawatase Yoshihiro, Toyoda Masashi, Okamura Kohji, Nakamura Ken-ichi, Nakabayashi Kazuhiko, Takada Shuji, Yamazaki-Inoue Mayu, Masuda Akira, Nasu Michiyo, Hata Kenichiro, Hanaoka Kazunori, Higuchi Akon, Takubo Kaiyo, Umezawa Akihiro
Аннотация
Ataxia telangiectasia is a neurodegenerative inherited disease with chromosomal instability and hypersensitivity to ionizing radiation. iPS cells lacking ATM (AT-iPS cells) exhibited hypersensitivity to X-ray irradiation, one of the characteristics of the disease. While parental ataxia telangiectasia cells exhibited significant chromosomal abnormalities, AT-iPS cells did not show any chromosomal instability in vitro for at least 80 passages (560 days). Whole exome analysis also showed a comparable nucleotide substitution rate in AT-iPS cells. Taken together, these data show that ATM is involved in protection from irradiation-induced cell death.
Предметные рубрики MeSH
AnimalsApoptosisAtaxia TelangiectasiaAtaxia Telangiectasia Mutated ProteinsBlotting, WesternCell DifferentiationCell ProliferationCells, CulturedCellular ReprogrammingChildChromosomal InstabilityExomeFluorescent Antibody TechniqueHigh-Throughput Nucleotide SequencingHumansImmunoenzyme TechniquesIn Situ Hybridization, FluorescenceInduced Pluripotent Stem CellsKaryotypingMaleMiceMice, Inbred NODMice, SCIDRNA, MessengerRadiation ToleranceReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTeratomaX-Rays