Diagnostic molecular pathology : the American journal of surgical pathology, part B · 2006
JC virus chromogenic in situ hybridization in brain biopsies from patients with and without PML.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Procop Gary W, Beck Rose C, Pettay James D, Kohn Debra J, Tuohy Marion J, Yen-Lieberman Belinda, Prayson Richard A, Tubbs Raymond R
Аннотация
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC polyoma virus. Electron microscopy and immunohistochemistry are the traditional methods of confirming the presence of the virus in brain biopsies from these patients. We studied the brain biopsies from 7 patients with PML and 6 patients without PML with chromogenic in situ hybridization (CISH) for the JC polyoma virus using a commercially available probe. The biopsies from the patients with the PML cases were proven to contain the JC polyoma virus by traditional and molecular methods. The CISH findings were compared with the known state of infection. All (7/7) of the biopsies from patients with PML were positive for the presence of polyoma virus by CISH, whereas the biopsies from patients without PML were uniformly negative. CISH seems to be a useful tool for the detection of the JC virus in brain biopsies from patients with PML, and is more accessible because a commercial probe is available.