International immunopharmacology · 2025
Low levels of CD39+ Tregs may predict poor outcome in children with sepsis.
СтатьяНазвание и аннотация: Перевод в очереди
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Ji Yanchun, Wang Chenhao, Wang Bo, Jin Bian, Zhuang Chengxi, Xu Jingting, Xu Feng, Zhao Yao, Niu Linlin, Fang Fang
Аннотация
OBJECTIVES: Sepsis is a life-threatening condition caused by a dysregulated immune response. This study aimed to explore immune dysregulation in pediatric sepsis and identify prognostic biomarkers for mortality prediction.
METHODS: Ninety-nine children with sepsis were followed for 28 days. Flow cytometry was used to analyze T cells, T-helper cells, B cells, natural killer (NK) cells, dendritic cells (DCs), and their subpopulations. Correlations between CD39+ regulatory T cells (Tregs), plasmacytoid DCs (pDCs), and transitional B cells with Phoenix Sepsis Score (PSS) were assessed. Receiver operating characteristic and Kaplan-Meier analyses were used to evaluate the prognostic value of CD39+ Tregs and pDCs. Cytokine levels were measured using a cytometric bead array.
RESULTS: CD39+ Tregs and transitional B cells were significantly lower in the nonsurvival and remote groups, while pDCs showed the opposite trend. CD39+ Tregs were negatively correlated with PSS, and pDCs were positively correlated. CD39+ Tregs below 12.45 % and pDCs above 0.96 % were both strong predictors of increased mortality risk. CD39+ Tregs maintained stable predictive performance within 72 h.
CONCLUSIONS: CD39+ Tregs and pDCs may serve as potential prognostic biomarkers in pediatric sepsis, with specific thresholds predicting increased mortality risk.
METHODS: Ninety-nine children with sepsis were followed for 28 days. Flow cytometry was used to analyze T cells, T-helper cells, B cells, natural killer (NK) cells, dendritic cells (DCs), and their subpopulations. Correlations between CD39+ regulatory T cells (Tregs), plasmacytoid DCs (pDCs), and transitional B cells with Phoenix Sepsis Score (PSS) were assessed. Receiver operating characteristic and Kaplan-Meier analyses were used to evaluate the prognostic value of CD39+ Tregs and pDCs. Cytokine levels were measured using a cytometric bead array.
RESULTS: CD39+ Tregs and transitional B cells were significantly lower in the nonsurvival and remote groups, while pDCs showed the opposite trend. CD39+ Tregs were negatively correlated with PSS, and pDCs were positively correlated. CD39+ Tregs below 12.45 % and pDCs above 0.96 % were both strong predictors of increased mortality risk. CD39+ Tregs maintained stable predictive performance within 72 h.
CONCLUSIONS: CD39+ Tregs and pDCs may serve as potential prognostic biomarkers in pediatric sepsis, with specific thresholds predicting increased mortality risk.