Orientational behaviors of liquid crystals coupled to chitosan-disrupted phospholipid membranes at the aqueous-liquid crystal interface.

In this study, we investigated the orientational behavior of liquid crystals (LCs) which is associated with the chitosan-disrupted phospholipid membrane at the aqueous/LC interface. The optical response of LCs changed from dark to bright after the transfer of an aqueous solution of chitosan onto the LC interface decorated with self-assembled monolayers of a negatively charged phospholipid, dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DOPG). The chitosan-lipid interactions induced a rearrangement of the membrane, and thus, resulted in an orientational transition of LCs from a homeotropic to a planar state, thereby triggering a dark-to-bright shift in the optical response. We observed that LCs exhibited a bright-to-dark shift after an aqueous solution of lysozyme was transferred onto the chitosan-disrupted membrane, which implied that an enzymatic reaction between lysozyme and chitosan took place. We found that the addition of bovine serum album (BSA) induced a bright-to-dark change in the optical response; while LCs remained to appear bright after the transfer of chymotrypsin onto the aqueous/LC interface. We then further examined the interactions between other polyelectrolytes and phospholipid membranes.