Triterpenoid saponins from Rubus setchuenensis roots and their anti-inflammatory activities in vitro.

Previously undescribed triterpenoid saponins (1-8), along with eight previously reported analogues (9-16), were isolated from an EtOAc extract obtained from the roots of Rubus setchuenensis. Structures were established based on HRESIMS, 1D and 2D NMR, as well as by literature comparisons. All compounds were isolated for the first time from the genus Rubus. Compounds 3-4, 6-8, and 12-15 displayed inhibitory activities against NO production in a RAW264.7 cell inflammation model induced by LPS. Moreover, 4, 6-8, and 12-13 exhibited inhibitory effects in a LPS-induced BV-2 cell neuroinflammation model.