Virology journal · 2013
Development and characterization of the replicon system of Japanese encephalitis live vaccine virus SA14-14-2.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Li Shi-Hua, Li Xiao-Feng, Zhao Hui, Deng Yong-Qiang, Yu Xue-Dong, Zhu Shun-Ya, Jiang Tao, Ye Qing, Qin E-De, Qin Cheng-Feng
Аннотация
BACKGROUND: Viral self-replicating sub-genomic replicons represent a powerful tool for studying viral genome replication, antiviral screening and chimeric vaccine development. Many kinds of flavivirus replicons have been developed with broad applications.
FINDINGS: The replicon system of JEV live vaccine strain SA14-14-2 was successfully developed in this study. Two kinds of replicons that express enhanced green fluorescent protein (EGFP) and Renilla luciferase (R.luc) were constructed under the control of SP6 promoter, respectively. Robust EGFP and R.luc signals could be detected in the replicon-transfected BHK-21 cells. Furthermore, the potential effects of selected amino acids in the C-terminal of envelope protein on replication were characterized using the replicon system.
CONCLUSIONS: Our results provide a useful platform not only for the study of JEV replication, but also for antiviral screening and chimeric vaccine development.
FINDINGS: The replicon system of JEV live vaccine strain SA14-14-2 was successfully developed in this study. Two kinds of replicons that express enhanced green fluorescent protein (EGFP) and Renilla luciferase (R.luc) were constructed under the control of SP6 promoter, respectively. Robust EGFP and R.luc signals could be detected in the replicon-transfected BHK-21 cells. Furthermore, the potential effects of selected amino acids in the C-terminal of envelope protein on replication were characterized using the replicon system.
CONCLUSIONS: Our results provide a useful platform not only for the study of JEV replication, but also for antiviral screening and chimeric vaccine development.