Orthoflavivirus nilense surveillance in the State of Piauí, northeastern Brazil.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Lobato Osmaikon Lisboa, Nogueira Tayná da Silva, Lima Tobias Emílio Tavares, Andrade Felipe José da Costa, de Macedo Marília Gabryelle Guimarães, Pereira Rayane de Souza, Xavier Joilson, Amorim Mariene Ribeiro, Barbosa Priscilla Paschoal, da Rocha Alex Sobrinho, Silva Silvokleio da Costa, Alcantara Luiz Carlos Junior, de Souza William M, Proenca-Modena José Luiz, Costa Érica Azevedo, Lima Neto Adelino Soares, Feitosa Lauro César Soares, Pires E Cruz Maria do Socorro, Silva Silvana Maria Medeiros de Sousa, Baêta Silvia de Araújo França, Vieira Marcelo Adriano da Cunha E Silva, Deem Sharon L, Catenacci Lilian Silva
Аннотация
OBJECTIVES: To investigate the presence of WNV in birds, mosquitoes, and equids in municipalities of Piauí.
METHODS: Collections were carried out following recommendations from the Ministry of Health of Brazil, in 11 municipalities (all with human cases or bird mortality), where biological samples were collected from birds, mosquitoes, and equids. The Viral RNA extraction was performed using a commercial kit, following the manufacturers' recommendations; samples were subjected to reverse transcription and polymerase chain reaction, with specific primers for WNV.
FINDINGS: 2,706 samples were collected (636 birds, belonging to 99 species; 420 equids, and 1,650 mosquitoes, grouped into 346 pools, totaling 18 species. No collected sample yielded a positive result, corroborating with other studies showing the difficulty of molecular detection of WNV in healthy animals, which may explain the non-detection, in addition to the delayed diagnosis in humans.
MAIN CONCLUSIONS: A local investigation involving suspected cases is still recommended in animals; however, in locations with late diagnosis in humans we suggest a serological survey of asymptomatic birds and equids.