Mn2O3 NPs-induced liver injury is potentially associated with gut microbiota dysbiosis in broiler chicken.

Mn2O3 nanometer particles(Mn2O3 NPs), a new material, is widely used in medicine, electrochemical sensing and energy storage fields. The widespread use of Mn2O3 NPs has caused health concerns, and it is necessary to clarify the toxic mechanism of Mn2O3 NPs exposure. Our findings showed that Mn2O3 NPs exposure could lead to liver histological abnormalities, mitochondrial dysfunction in liver, as well as mitochondrial-mediated apoptosis, autophagy and mitochondrial dynamics disorder, and eventually lead to liver injury. At the same time, the ileal epithelium suffered physiological damage and inflammation after Mn2O3 NPs exposure, and the expression levels of genes and proteins related to intestinal barrier function (MUC1 ZO-1 Claudin1 and Occludin) were significantly down-regulated. Meanwhile, 16s sequencing analysis of intestinal bacteria showed that Mn2O3 NPs exposure caused significant changes in intestinal flora abundance. The Firmicutes/Bacteroidetes ratio increased, and the abundance of probiotics (Bacteroides, Bifidobacterium, Faecalibacterium) decreased, while the abundance of harmful bacteria (Streptococcus, Enterococcus, Pseudomonas) increased. The changes in these microflorae may potentially impact the development of liver injury. Altogether, these results provide novel insights into the potential mechanism of Mn2O3 NPs related hepatotoxicity induced by gut microbiota via the gut-liver axis, and contribute to a better interpretation of the health impact of Mn2O3 NPs.