Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion · 2012
[CSF RT-PCR standardization in pediatric patients with enteroviral central nervous system infection].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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García-Elorriaga Guadalupe, Esparza-García Amalia, Méndez-Rojas Claudia, del Rey-Pineda Guillermo, González-Bonilla César
Аннотация
OBJECTIVE: We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years.
MATERIAL AND METHODS: RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system.
RESULTS: 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments.
CONCLUSIONS: Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.
MATERIAL AND METHODS: RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system.
RESULTS: 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments.
CONCLUSIONS: Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.