Journal of cancer research and therapeutics · 2018
Anticancer potential of leaf and leaf-derived callus extracts of Aerva javanica against MCF-7 breast cancer cell line.
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Kamalanathan Desingu, Natarajan Devarajan
Аннотация
BACKGROUND: Aerva javanica is an exotic and medicinal plant in India.
AIM OF THE STUDY: The main goal of this study was to evaluate the antiproliferative properties of leaf and leaf-derived callus extracts against human breast cancer cell line MCF-7.
METHODS: The plant parts were sequentially extracted with hexane, chloroform, ethyl acetate, acetone, and methanol. The extract was concentrated to yield the crude extract, which was tested for anticancer potentials. The anticancer potential of cytotoxic extracts was determined by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays in human breast cancer cell lines (MCF-7).
RESULTS: All the tested extracts showed significant antiproliferative activities in a concentration- and time-dependent manner. The inhibitory concentration of extract was tested against target cell line, and the results show in vitro leaf of A. javanica has higher inhibitory effect against the tested cancer cells at lower concentration (about 11.89 and 22.45 μg/ml) followed by other samples extracts.
CONCLUSION: The results of the present study conclude in vitro plant sample having more potent anticancer property and support the need of further studies to isolate potential anticancer drug with cancer cell-specific cytotoxicity.
AIM OF THE STUDY: The main goal of this study was to evaluate the antiproliferative properties of leaf and leaf-derived callus extracts against human breast cancer cell line MCF-7.
METHODS: The plant parts were sequentially extracted with hexane, chloroform, ethyl acetate, acetone, and methanol. The extract was concentrated to yield the crude extract, which was tested for anticancer potentials. The anticancer potential of cytotoxic extracts was determined by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays in human breast cancer cell lines (MCF-7).
RESULTS: All the tested extracts showed significant antiproliferative activities in a concentration- and time-dependent manner. The inhibitory concentration of extract was tested against target cell line, and the results show in vitro leaf of A. javanica has higher inhibitory effect against the tested cancer cells at lower concentration (about 11.89 and 22.45 μg/ml) followed by other samples extracts.
CONCLUSION: The results of the present study conclude in vitro plant sample having more potent anticancer property and support the need of further studies to isolate potential anticancer drug with cancer cell-specific cytotoxicity.