Clinical nutrition (Edinburgh, Scotland) · 2008
Inhibitory effect of olive oil on fibrosis induced by carbon tetrachloride in rat liver.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Fang Hsun-Lang, Lai Jinn-Tsyy, Lin Wen-Chuan
Аннотация
BACKGROUND & AIMS: In this study, the inhibitory effect of olive oil on liver fibrosis induced by carbon tetrachloride (CCl(4)) has been investigated in rats.
METHODS: Rats were divided randomly into four groups: control, CCl(4), and two olive oil groups. Except for rats in the control group, all rats were orally administered CCl(4) twice a week for 8 weeks. Rats in the olive oil groups were treated daily with olive oil (2 or 10 ml/kg) through gastrogavage for the entire experimental period.
RESULTS: RT-PCR analysis showed that CCl(4) increased the hepatic mRNA expressions of lipopolysaccharide binding protein, CD14, Toll-like receptor-4, NADPH oxidase, nuclear factor-kappa beta, collagen (alpha1) (I), collagen (alpha1) (III), and transforming growth factor beta1. The expression of these mRNAs could be decreased by olive oil treatment. In addition, Western blot analysis also supported these results. CCl(4)-induced liver damage, as characterized by the increase in hepatic malondialdehyde and hydroxyproline levels. Olive oil treatment decreased the hepatic malondialdehyde and hydroxyproline levels. Histological evaluations showed that olive oil could attenuate the liver fibrosis, necrosis, and expression of smooth muscle alpha-actin that are induced by CCl(4).
CONCLUSION: It is speculated that the phenolic compounds in olive oil significantly reduced CCl(4)-induced hepatic fibrosis in rats.
METHODS: Rats were divided randomly into four groups: control, CCl(4), and two olive oil groups. Except for rats in the control group, all rats were orally administered CCl(4) twice a week for 8 weeks. Rats in the olive oil groups were treated daily with olive oil (2 or 10 ml/kg) through gastrogavage for the entire experimental period.
RESULTS: RT-PCR analysis showed that CCl(4) increased the hepatic mRNA expressions of lipopolysaccharide binding protein, CD14, Toll-like receptor-4, NADPH oxidase, nuclear factor-kappa beta, collagen (alpha1) (I), collagen (alpha1) (III), and transforming growth factor beta1. The expression of these mRNAs could be decreased by olive oil treatment. In addition, Western blot analysis also supported these results. CCl(4)-induced liver damage, as characterized by the increase in hepatic malondialdehyde and hydroxyproline levels. Olive oil treatment decreased the hepatic malondialdehyde and hydroxyproline levels. Histological evaluations showed that olive oil could attenuate the liver fibrosis, necrosis, and expression of smooth muscle alpha-actin that are induced by CCl(4).
CONCLUSION: It is speculated that the phenolic compounds in olive oil significantly reduced CCl(4)-induced hepatic fibrosis in rats.
Предметные рубрики MeSH
AnimalsBlotting, WesternBody WeightCarbon TetrachlorideCatalaseCollagenGlutathione PeroxidaseHydroxyprolineImmunohistochemistryLipid PeroxidationLiver CirrhosisMaleMalondialdehydeOlive OilPlant OilsRandom AllocationRatsRats, WistarReverse Transcriptase Polymerase Chain ReactionSuperoxide DismutaseTransforming Growth Factor beta1