Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.

In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties. The bonding strength between coatings was studied by scratch method. The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests. The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility. In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property. All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF). After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution. The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.