Comparative proteomics of kidney samples from puffer fish Takifugu rubripes exposed to excessive fluoride: an insight into molecular response to fluorosis.

To investigate comparative proteomics of the pufferfish kidney exposed to excessive fluoride, the authors randomly put 16 fish into the control and treated groups that were raised in softwater alone (F(-) = 0.4 mg/L) or with sodium fluoride of 35 mg/L for 3 days, respectively. Then proteins of the fish kidneys were profiled by two-dimensional electrophoresis, and the matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF MS) was applied to identify the spots on the gel with altered densities. On average, 547 and 516 protein spots were detected in the control and the treated groups, respectively. Among them, 32 protein spots showed significant alteration (p < 0.05) between the fluoride-treated and the control groups, and 22 differentially expressed protein spots were identified by MALDI TOF-TOF MS. Consistent with their previously annotated functions, these proteins appear to be involved in the biological functions associated with fluorosis. These results will greatly advance one's understanding of the effects of fluoride exposure on the physiological and biochemical functions of takifugu kidney as well as the toxicological mechanism of fluoride-causing fluorosis in both fish and humans.